Publications
Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis
Amanda Mixon Blackwell, Yasaman Jami-Alahmadi, Armiyaw S. Nasamu, Shota Kudo, Akinobu Senoo, Celine Slam, Kouhei Tsumoto, James A. Wohlschlegel, Jose M. M.Caaveiro, Daniel E. Goldberg, Paul A. Sigala
eLife, 2024, Aug. 27.
Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites
Kade M. Loveridge and Sigala PA
Proc Natl Acad Sci U S A. 2024 Nov 5;121(45):e2411631121
University of Utah Highlight: Discovery of Critical Iron-Transport Protein in Malaria Parasites Could Lead to Faster-Acting Medications
Biogenesis of cytochromes c and c1 in the electron transport chain of malaria parasites
Aldo E. García-Guerrero, Rebecca G. Marvin, Amanda Mixon Blackwell, Paul A. Sigala
ACS Infect Dis. 2024 Oct 31.
The interdependence of isoprenoid synthesis and apicoplast biogenesis in malaria parasites
Megan Okada and Paul A Sigala
PLoS Pathogens. 2023 Oct. 26;19(10):e1011713
Tanya J. Espino-Sanchez
Elife. 2023 Sept. 5; 12:e92403.
Direct tests of cytochrome c and c1 functions in the electron transport chain of malaria parasites
Tanya J. Espino-Sanchez*, Henry Wienkers*, Rebecca G. Marvin*, Shai-anne Nalder, Aldo E. García-Guerrero, Peter E. VanNatta, Yasaman Jami-Alahmadi, Amanda Mixon Blackwell, Frank G. Whitby, James A. Wohlschlegel, Matthew T. Kieber-Emmons, Christopher P. Hill, and Paul A. Sigala
PNAS. 2023 May 1;120 (19) e2301047120.
Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow
Haidong Feng, Dhruv Patel, Jules J. Magda, Sage Geher, Paul A. Sigala, and Bruce K. Gale
ACS Omega. 2022 7: 41759–41767.
Critical role for isoprenoids in apicoplast biogenesis by malaria parasites
Okada M, Rajaram K, Swift RP, Mixon A, Maschek JA, Prigge ST, Sigala PA
Elife. 2022 Mar 8;11:e73208.
Divergent Acyl Carrier Protein Decouples Mitochondrial Fe-S Cluster Biogenesis from Fatty Acid Synthesis in Malaria Parasites
Seyi Falekun, Jaime Sepulveda, Yasaman Jami-Alahmadi, Hahnbeom Park, James A. Wohlschlegel, Sigala PA
Elife. 2021 Oct 6;10:e71636.
Doxycycline has Distinct Apicoplast-Specific Mechanisms of Antimalarial Activity
Megan Okada, Ping Guo, Shai-anne Nalder and Sigala PA
Elife. 2020 Nov 2;9:e60246.
Plasmodium heme biosynthesis: To be or not to be essential?
Goldberg DE and Sigala PA
PLoS Pathog. 2017 Sep 28;13(9):e1006511.
In-Cell Enzymology To Probe His–Heme Ligation in Heme Oxygenase Catalysis
Sigala PA, Morante K, Tsumoto K, Caaveiro JM, and Goldberg DE
Biochemistry. 2016 Aug 30;55(34):4836-49.
Deconvoluting heme biosynthesis to target blood-stage malaria parasites
Sigala PA, Crowley JR, Henderson JP, Goldberg DE
Elife. 2015 Jul 14;4:e09143.
The heme biosynthesis pathway is essential for Plasmodium falciparum development in mosquito stage but not in blood stages
Ke H*, Sigala PA*, Miura K, Morrisey JM, Mather MW, Crowley JR, Henderson JP, Goldberg DE, Long CA, Vaidya AB
J Biol Chem. 2014 Dec 12;289(50):34827-37.
The peculiarities and paradoxes of Plasmodium heme metabolism
Sigala PA, Goldberg DE
Annu Rev Microbiol. 2014; 68:259-78. [Review]
Direct tests of enzymatic heme degradation by the malaria parasite Plasmodium falciparum
Sigala PA, Crowley JR, Hsieh S, Henderson JP, Goldberg DE
J Biol Chem. 2012 Nov 2;287(45):37793-807.
Fighting Malaria with Light
Funding
Patent
Combination artemisinin and chemiluminescent photodynamic therapy
Prior Publications
Determination of Hydrogen Bond Structure in Water versus Aprotic Environments To Test the Relationship Between Length and Stability
Sigala PA, Ruben EA, Liu CW, Piccoli PM, Hohenstein EG, Martínez TJ, Schultz AJ, Herschlag D.
J Am Chem Soc. 2015 May 6;137(17):5730-40.
Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site
Sigala PA, Fafarman AT, Schwans JP, Fried SD, Fenn TD, Caaveiro JM, Pybus B, Ringe D, Petsko GA, Boxer SG, Herschlag D.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):E2552-61.
Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase
Fafarman AT, Sigala PA, Schwans JP, Fenn TD, Herschlag D, Boxer SG.
Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):E299-308.
Hydrogen bonding in the active site of ketosteroid isomerase: electronic inductive effects and hydrogen bond coupling
Hanoian P, Sigala PA, Herschlag D, Hammes-Schiffer S.
Biochemistry. 2010 Dec 7;49(48):10339-48.
Decomposition of vibrational shifts of nitriles into electrostatic and hydrogen-bonding effects
Fafarman AT, Sigala PA, Herschlag D, Boxer SG.
J Am Chem Soc. 2010 Sep 22;132(37):12811-3.
Dissecting the paradoxical effects of hydrogen bond mutations in the ketosteroid isomerase oxyanion hole
Kraut DA, Sigala PA, Fenn TD, Herschlag D.
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1960-5.
Hydrogen bond dynamics in the active site of photoactive yellow protein
Sigala PA, Tsuchida MA, Herschlag D.
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9232-7.
Hydrogen bond coupling in the ketosteroid isomerase active site
Sigala PA, Caaveiro JM, Ringe D, Petsko GA, Herschlag D.
Biochemistry. 2009 Jul 28;48(29):6932-9.
Testing geometrical discrimination within an enzyme active site: constrained hydrogen bonding in the ketosteroid isomerase oxyanion hole
Sigala PA, Kraut DA, Caaveiro JM, Pybus B, Ruben EA, Ringe D, Petsko GA, Herschlag D.
J Am Chem Soc. 2008 Oct 15;130(41):13696-708.
Do ligand binding and solvent exclusion alter the electrostatic character within the oxyanion hole of an enzymatic active site?
Sigala PA, Fafarman AT, Bogard PE, Boxer SG, Herschlag D.
J Am Chem Soc. 2007 Oct 10;129(40):12104-5.
Testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole
Kraut DA, Sigala PA, Pybus B, Liu CW, Ringe D, Petsko GA, Herschlag D.
PLoS Biol. 2006 Apr;4(4):e99.